This course will focus on the theoretical and experimental aspects of quantitative ultrasound (QUS) methods based on the backscattered coefficient, envelope statistics, and ultrasound attenuation. QUS methods permit quantitative soft-tissue microstructure quantitively and in detail and have a long history of success in numerous organ systems. Attendees will become familiar with state-of-the-art methods and will be able to apply them in their own research. The course will also briefly review recent QUS successes from recent literature.